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>Hello,
>I culture brain slices on millipore inserts. I plan to do some real time
>imaging on a confocal (for 24-48hrs). My problem lies with the insert. The
>confocal Im using has the objectives underneath the sample which means that
>it has to image through the insert membrane. We have found that the
>microscope can not do this. The inserts have small 3mm feet and a top
>height of 5mm. I could try inverting the whole insert but I think the media
>would then have to go above the insert which may cause the slice to fall
>off, also it would probably make a seal between the insert and the dish
>making my slices hypoxic. Somebody has given me some matrex dishes which
>have a coverslip bottom and ideally I need to place my slices on them and
>cover them with media. Im not sure if one side of the slice needs to be
>exposed to facilliate gas exchange - any comments on this and inserts would
>also be appreciated, has anyone tried it before?.
>Kate.x.

Kate

If you could call me at Bioptechs I would be happy to discuss
solutions to that problem.

Dan

--
Dan Focht
Bioptechs
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