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Hello,
I am helping someone determine whether their protein is on the
membrane, inside the cell, or both.  the living cells (I don't
remember what kind they are) are grown on cover slips and are very
flat.  It is difficult to tell.  they are thinking of counterstaining
with a cytoplasmic dye.  Several come to mind, like rhodamine 123 for
mitochondria, or a Cell Tracker dye.  conversely one could try to
stain the cell membrane.
Does anyone have any suggestions?  Would it be OK to use cells that
are rounding up? They are the brightest but I'm worried their
membranes may be compromised.
Thanks in advance.
Carol
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Carol Bayles
Microscopy,  Imaging & Fluorimetry (MIF)
Bioresource Center
160 Biotech Bldg
www.brc.cornell.edu

Confocal and Multiphoton Microscopy
Nanobiotechnology Center
D21-H Clark Hall
www.nbtc.cornell.edu

Cornell University
Ithaca NY 14853
607-254-4860
607-254-4847  fax