CONFOCALMICROSCOPY Archives

January 2003

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Subject:
uneven DIC on Leica Sp2
From:
David Knecht <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 23 Jan 2003 19:23:25 -0500
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Our Leica SP2 has a problem in which the DIC image is uneven, with the
upper left and lower right quadrants much brighter than the rest of the
image.  The problem disappears if you remove the analyzer from the
light path.  It is most evident at high mag (100x oil) and also
disappears if you zoom  the field of view.  Any ideas on what is
causing the problem?  Thanks- Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

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