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I assume you want to label cell nuclei and not DNA in vitro.  For both purposes Molecular Probes has a series of fluorophores that bind to nucleic acid.  Some are intended to be membrane permeant and are useful for live cells, others are meant to be used on fixed cells.  I have used one of the "membrane impermeant" labels (YO-PRO-1, using 488 laser excitation and FITC filter set) in live tissue and had very good label.  There are many others to choose from. 
Roger 

-----Original Message-----
From: Louis Villeneuve [mailto:[log in to unmask]]
Sent: Thursday, February 06, 2003 1:59 AM
To: [log in to unmask]
Subject: DNA staining


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Hi everyone,

I am looking for staining the DNA of a mouse ovocyte.  It is for dual
labeling and my 1st fluorochrome is Cy3.  Propiduim iodine and Ethiduim
bromide are in the range of Cy3 so I can't use them.   I cannot use UV's
suitable fluorochrome because I don't have the laser for it.  So I am
looking for DNA markers emitting in the range of FiTC or Cy5 for dual
labeling.

Can't you help me...

Thanks

Louis Villeneuve
Louis Villeneuve
Technicien de recherche- microscope confocal

Institut de Cardiologie de Montreal- Centre de recherche
5000 Belanger Est
Montreal (Quebec)
H1T 1C8

tel: 514-376-3330-3511
fax: 514-376-1355
email: [log in to unmask]