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A debate is brewing as to whether including multiphoton capability on our soon-to-be purchased confocal scope is necessary. 

Given the following parameters: 1) core facility; 2) need to triple label from UV to Red and all points in between; 3) mostly fixed tissue with some potential for cell culture work; just how often would the ability to tune (we're talking the new automated Coherent Chameleon here) excitation really make the world of difference?

I am currently of the opinion that limiting bleaching throughout the entire section, selective excitation and future flexability warrant the $$. Yet I'm suspicious enough to forsee that 2p might not have a worthy cost to benefit ratio.

Opinions?

Greg Holmes
Pennington Biomedical Research Center