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Dear listers,

a client of our facilities wants to perform the following experiment:

-transfect fluorescently labeled dna using different electroporation
methods.
-observe distribution of the dna by microscopy and follow up dna entry
into cell nuclei.

The transfection is taking place in a cuvette, therefor the main
technical problem is to get the cells as soon as possible after the
transfection into a microscopy compatible form, i.e onto a coverslip.

Any of you did a similar experiment? How to proceed after the
transfection? Use Cytospin? Low melting agarose embedding? Other
strategy?

Thanks for your comments,
Jens


--

Dr. Jens Rietdorf
EMBL, Meyerhofstr.1,
D-69117 Heidelberg,
Cell Biology/Biophysics Program,
Advanced Light Microscopy Facility
Phone ++49(0)6221 387-467 FAX-306
http://www.embl-heidelberg.de/~rietdorf/index.html
http://www.embl-heidelberg.de/ExternalInfo/almf/htdocs/almf_website/index.html