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June 2003

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Subject:
Re: Blur image on transwell
From:
Kathy Spencer <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 17 Jun 2003 10:48:20 -0700
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>We also were interested in looking a junctions in cells grown on
>Transwells. We took a 4mm dermatone punch (Accuderm) to remove the
>Transwell filter from the well (after staining), then mounted the filters
>on slides, with coverslips. Worked very well, with no tearing of the
>filter or cells (if you used a very sharp, fresh dermatome).

         Kathy




Kathryn S.R. Spencer, Ph.D.
The Scripps Research Institute
10550 N. Torrey Pines Road
ICND202
La Jolla, CA 92037
(858) 784-8437

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