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Hello Jessica,

We use Rhodamin 123 often.
It is simple to use:
We have a stock solution of 100 µM in water and keep this in small
quantities in the freezer.
-Add 1 µM final concentraion to your live cells for 15 minutes.
-Wash two times.
-Look at the microscope or FACS.


Succes,

Hans



On 4 Jun 2003, at 3:51, Jessica Sample wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Does anyone have a protocol for staining cells (isolated
> cardiomyocytes) with either JC-1 or Rhodamine 123 in order to measure
> the mitochondrial membrane potential. I am also thinking about using
> mitotrackers, can anyone recommend which one to use (with Rho123 or
> Jc-1). Thanks


Hans de Bont
Leiden/Amsterdam Center for Drug Research
Leiden University
Einsteinweg 55
2333 CC  Leiden
The Netherlands
Tel: 071-5276225