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Glycerine-Solution is sold by Leica, Cat No 11513872, the Ne@37° is 1.460!
But 85 % dilution is about right and there is still a correction ring to adjust for precise imaging condition.

Urs

At 11:49 16.06.2003 +0100, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>We have purchased the new x63 glycerol objective for use at 37C from Leica
>but they don't sell the immersion medium. The service engineer says to use a
>85% dilution can anyone advise me if this is correct and if I add a
>preservative to the solution, if so what?
>
>From:   Mr Stephen Pountney
>Tel:  +44 (0)118 378 8896 (direct)
>Tel:  +44 (0)118 987 5123 ext 8896
>Fax: +44 (0)118 931 0180
>e-mail    [log in to unmask]
>
>The University of Reading
>School of Animal & Microbial Sciences
>Whiteknights, PO Box 228, Reading RG6 6AJ