Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

One more in the list ! We had exactely the same problem Larry and Xuejun

described. Several of our oil objectives were crashed and we thought all
the
time that some careless user or our live cell chamber (with metall
parts)
would be the reason.
However, there were no sharp metall edges with could scratch in such a
way
the top metall cover and the front lens of an objective just in the
center.
We heard from an english group having the same problem and than inquired

with Zeiss.
The company now offered us to get the damaged objectives repaired and to

change the bottom side of the stage in a way that it  wont damage the
nearby
objectives and there are no sharp edges anymore (in principle by
drilling a
bigger hole at this position).

Best wishes,

Anje

***************************************
Dr. Anje Sporbert

AG Leonhardt/Cardoso
Max-Delbrueck-Center for Molecular Medicine
Franz-Volhard-Clinic
Wiltbergstr. 50
13125 Berlin
Germany


tel. 030 9417 2260
fax: 030 9417 2336
email: [log in to unmask]

***************************************

Larry Tague wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Wow!  We have had this same problem, and we always thought that we
> might just have a careless user who forced the high NA 63X objective
> ($4000 repair) into something hard.  We could never figure out what
> that might have been.  Now we know that someone was using the 10x
> objective while the 63X objective was in position to strike the back
of
> the stage if they tried to focus the 10x too high .  We have already
> sent a note to our Zeiss rep letting them know that we cannot afford
to
> fix another lens.  Please let us know if someone has a fix for this
> problem.
>
> Larry
>
> On Friday, July 25, 2003, at 03:19 PM, xjsun wrote:
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Hello everyone,
> >
> > After 2 lenses damaged, we finally find out how it happened. This
> > applies to specific combination of Zeiss Axiovert 200 and Zeiss
> > motorized scanning stage.
> >
> > When the stage is moved to far left (or right) position, a
supporting
> > frame under stage will hit the objective (high NA) next to the
> > currently
> > used lens. The final result is that there will be a dent right in
the
> > middle of the objective. This will lead to broken seal of the lens.
> >
> > What a wonderful piece of engineering Zeiss produced! Of course,
> > repairing the lens costs a fortunate and takes forever (6 months
now).
> >
> > Raising the stage will reduce the chances but does not solve the
> > problem.
> >
> > Hope this would not happen to anyone else and I am also curious to
know
> > if anyone has lens damaged in similar way with such combination of
> > scope
> > and stage.
> >
> > Best wishes,
> >
> > Xuejun
> >
> > --
> > Xuejun Sun, Ph.D.
> > Dept. Exp. Oncology
> > Cross Cancer Institute
> > 11560 University ave.
> > Edmonton Alberta T6G 1Z2
> > Canada
> >
> > Phone:  (780) 432-8898 (office)
> >        (780) 432-8468 (lab.)
> > Fax:    (780) 432-8425