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Olaf,
For a CCD addition on another image capture device, by chance we found that
when we changed a network card on our computer, the horizontal lines
appeared similarly as you describe.  Removal of the card immediately cleared
the problem.  Apparently, some electronic interference on the CPU bus caused
this problem which was not immediately visible on the screen, but became
more visible when processing for details on the image. Consider all hardware
changes to your system while troubleshooting.
Tom

----- Original Message -----
From: "Olaf Selchow" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, August 08, 2003 8:12 AM
Subject: Leica SP2 with 2p setup (Coherent Mira 900)


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello confocal colleagues...
>
> I have a problem with our Leica SP2 system: The images show thin
> dark, horizontal stripes. Sometimes more and sometimes less. I
> couldn't figure out yet when the stripes are stronger and when not.
> Sometimes they seem to have gone completely. It looks a little like a
> phase-problem with the line scans. But I couldn't fix it by adjusting
> the phase-correction.
>
> One more hint I got is the following: The stripes were seen the first
> time after the 2Photon system (IR laser Coherent Mira 900, EOM and
> all the extra bits and pieces) has been added to the standard SP2.
> Unfortunately this has been before I joined the lab. So I can not
> confirm this myself. Before that the images were fine (that's what
> the older colleagues here in the lab say).
>
> Independent from the 2P system I thought that the stripes could come
> from some noise on the electricity supply. We're a big institute and
> I don't know what other equipment is used in the building... and as I
> said I couldn't correlate it with any external parameter like
> daytime, weekday or something else.
>
> The images are sufficiently good quality to interpret. But they are
> no good for publication....
>
> Has anybody had such a problem before and/or have an idea about how
> to solve it?
> I'd like to avaid to call (and pay) the Leica service when it's maybe
> just a very easy thing that I can do myself.
>
> Thanks in advance for your help!
>
> Olaf
>
> --
>
>   --------------------------------------------
> | Dr. Olaf Selchow                           |
> | SFB 495 Z1 - Central Microscopy Facility   |
> | Institute for Cell Biology und Immunology  |
> | University of Stuttgart                    |
> | Allmandring 31, 70569 Stuttgart, Germany   |
> | T: +49 711 685 8209, Fax: +49 711 685 7484 |
> | email: [log in to unmask]    |
>   --------------------------------------------