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Olaf,
Thanks for the pics.
Though I know this pattern in X-T scans, I personally never
observed it in X-Y images.
It looks like 50 Hz intensity modulation. If you make a
vertical plot over the stripe pattern. Combine the period of
the sine wave with the scan speed to see whether it is
really 50 or 100Hz.
I do exclude the Mira. Once it falls out of mode-lock I
doubt it would come back by itself at 50Hz. The argon laser
might be the cause, but not if you see the pattern with the
Mira as well.
Interestingly, a 50 Hz interference is not necessarily
synchronous with the Galvo scanner, so I would expect rather
diagonal stripes than only horizontal. On the other hand,
intensity changes only in Y direction (vertical) in your
images and over several scan lines. So I don't think it's
the Y-Galvo mirror. Could it be vibration of the
coverslip/focus in Z direction? This excluded I would check
more "upstream", i.e. AOTF, AOBS (if present), PMT, frame
grabber.
Good luck,
Franco
Olaf Selchow wrote:
> Dear Ian, Franco, Sudipta, John, James, Tom... ;-)
>
> thanks for your answers!
>
> I checked out the PMTs and the lighting of the room. That's definitely
> not the reason. I have them in all PMTs and independent of room lighting
> (the room lighting is always the same anyway...)
>
> The hardware components I could not check yet... and I'll try to knock
> on the doors of the onther labs during the next days and try to find out
> what equipment they're running.
>
> The most supicious thing, however, is the Galvano-stage (object
> carriage) that Ian pointed out. I have to think about how I can check
> this. What did you do to fix it, Ian? Change the stage?
>
> Attached to that mail I send you two images that are not very nice from
> the biological point of view but clearly show the stripes. (I croped the
> images because of file size... but the cut edges of theimages were
> pretty black anyway...)
>
> Thanks very much!
>
> Olaf
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