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Dear Roberto,

Phagocytosis is a very temperature dependant process as cellular
membranes are involved. One of the best ways of fully controlling the
temperature of the cells at 37deg over an extended period on the
microscope is to fully enclose the microscope (or at least the
microscope stage and objectives) with an insulated cover. These can be
purchased commercially, or you can make your own using insulated bubble
plastic and a small egg incubator heater.

A heated imaging chamber (for example, the Bioptechs Delta T dish) with
a microscope objective heater can also be used, but for critical
temperature control over an extended period an enclosed microscope is
much better.

Good luck,

Regards, Alan.

BIOCON (publishers of the manual "Confocal Microscopy for Biologists",
mailto:[log in to unmask])
7 Walhalla Drive                 Phone: 61 3 9876 9822    FAX: 61 3 8660
2290
Ringwood East VIC 3135        Director: Dr. Alan R. Hibbs
Australia


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Roberto Caricchio
Sent: Friday, 22 August 2003 1:49 PM
To: [log in to unmask]
Subject: Re: Help

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello everybody,

I need to run some experiments of phagocytosis and image the uptake by
phagocytic cells. Of course I will need to keep the dish temperature at
37 degrees and I do not have a temperature controlled chamber. I am
willing to buy one and any suggestion is greatly appreciated.

Thank you very much

Roberto
_______________________
Roberto Caricchio, MD
Research Assistant Professor
Division of Rheumatology
University of Pennsylvania
751 BRB II/III, 421 Curie Blvd.
Philadelphia PA 19104
Office 215-573-4394
FAX   215-573-7599
email: [log in to unmask]

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