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Antoine, I assume you mean that phalloidin-Alexa 
633 didn't work because it gave no staining, not 
simply because it was dim.

Antoine has reminded me of something I have been 
wanting to ask for a long time and I am guessing 
that someone at Molecular Probes probably knows, 
which is, what are the quantum yields of the 
different Alexa probes. I personally have only 
used the Alexa 488 and 546 probes that have 
already been conjugated to a protein when 
purchased. The Alexa 488 appeared much brighter 
and camera readouts appeared to indicate that it 
was in fact brighter. However, this was entirely 
evaluated without calibration of anything.

If anyone has any quantum efficiency data for the 
family of Alexa dyes free in solution and/or 
bound, I would really appreciate the information. 
I could always measure it myself but its not 
convenient at the moment, as my lab is 
spectrofluorometer free. A reference would be 
nice, also.

Regards,
Mario


>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi everyone,
>Just a tip.
>If you have to choose between Phalloidin Alexa 
>488 and Phalloidin Alexa 633, in
>our hand, for similar experimentals conditions, 
>phalloidin Alexa 488 works wlle
>and phalloidin Alexa 633 doesn't.
>Bye
>
>--
>Antoine Blanc, Research Associate
>Ecole Polytechnique de Montreal
>Chemical Engineering, Mike Buschmann Laboratory
>CP 6079, succ. Centre-ville
>Montréal Qc, Canada H3C 3A7
>Tel.:514-340-4711 ext.:3212,3336,3337
>FAX:514-340-4159
>secrétariat: 514-340-4711 ext.:4984


--
_________________________________________________________
Mario M. Moronne, Ph.D.
NanoMed Technologies LLC
President and CTO
ph (510) 528-2400
FAX (510) 528-8076
1561 Posen Ave
Berkeley, CA
94706

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