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Boyko, Vitaly wrote:

 > I wonder if there are laser(s) available with a line at 584+/-10 nm?
 >

We recently added a 594nm HeNe to our BioRad MRC-1024. What may of general
interest to the group is that the Melles Griot yellow HeNe has the same
dimensions as the Melles Griot green HeNe. In fact, we are using the
same hardware and fiber coupling as for the green HeNe. However, you
will need to change the first dichroic to reflect 594nm instead of 543nm.

For BioRad MRC-1024 users: we bought our yellow HeNe from NMX Consulting
(search the confocal list for their post) and after 10-20 hours of use it
is putting out about 2.5-2.6 mW. (It started at 2.8mW, but it is common for a
new laser to "break in" and then settle to a new steady state.)
At the input to the scanhead after the fiber
we get about 1.6-1.7 mW, and at the output of the scanhead, about 0.16mW. The
reason for the big loss in the scanhead is that we are using the B1 beamsplitter
as the first dichroic, which is a  very bad choice. When we get a new dichroic
I expect to get a lot more light out of the scanhead. Currently. we have enough
light for detection, but we would obviously like to have more light for
acceptor photobleaching.

--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-5340697