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I note with interest that Molecular Probes has discontinued their prepared
coverslips using long wavelength Alexa phalloidin due to stability issues.
Apparently there are problems here even under the best circumstances.
----- Original Message -----
From: "hpadams" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Monday, December 22, 2003 1:07 PM
Subject: phalloidin 633


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> It maybe a mounting media compatibility issue. We recently found that
> phalloidin 543 when used with vectashield bleached rapidly. So we switched
to
> phalloidin 594 and it worked great. We switched after talking to Molecular
> Probes who stated (according to the person who talked to them... so this
is
> secondhand) that phalloidin 543 did not work with Vectashield. Has anyone
> notice this?
>
> Hank Adams
> Manager
> Microscopy Core
> Molecular Genetics
> UT MD Anderson Cancer Center
> Houston, Tx 77030
> 713 792-7899
>