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Dear Colleagues,

I would like to run some real time phagocytosis of apoptotic cells
essays. I was wondering if any of you could share his/her expertise in
dyeing the cytoplasmic membrane of the phagocytic cell. I have two
problems to solve for this matter: 1) the apoptotic cells are already
fluorescent, they express eGFP, so the dye has to be far from eGFP, 2)
the phagocytic cells are capable of pinocytosis and I would like to
exclude that possibility, so probably a dye working at 4 degrees would
do it.

Any insight will be very much appreciated.

Sincerely

Roberto

_______________________
Roberto Caricchio, MD
Research Assistant Professor
Division of Rheumatology
University of Pennsylvania
751 BRB II/III, 421 Curie Blvd.
Philadelphia PA 19104
Office 215-573-4394
FAX   215-573-7599
email: [log in to unmask]