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We are using a Zeiss LSM 510 META to perform linear unmixing for eCFP and
eGFP.  When unmixing a sample with ONLY eCFP using spectra for both
fluorochromes, the result includes a low intensity grainy textured signal for
eGFP.  Does anyone know a way to eliminate this erroneous signal?  This
appears using online fingerprinting as well as linear unmixing with only
advanced linear unmixing checked for the options.

Thanks in advance for any suggestions.

Tami Casavan
UND Medical School