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Hi All,
The Leica software does have an ROI scanning functionality, the only
problem is that the button(s) that control it are usually not present on
the default GUI (graphic user interface).  Go to Tools-->Customize.
This opens a window with a list of different commands.  Select 'Basic
Scan' from the list and a number of buttons should appear in the right
part of the window.  The two that you want are the 'ROI' and 'Config'
buttons, just drag them onto the main GUI where you want them to be.
The 'ROI' button lets you define the areas you want to scan, while the
'Config' button lets you specify which laser lines you want to use in
each area (i.e. in ROI-1 you want to use 488 and 568, while in ROI-2 you
want only 488 etc.).
This feature is very useful for not only FRAP and FRET analyses, but
also for checking to see if using a particular laser power is bleaching
a sample; the AOTF blanks the laser light outside of the ROI so those
parts of the sample are not exposed to the excitation light.  Any
confocal with an AOTF should be able to do this type of scanning.
Cheers,
Russell

Jill Pflugheber wrote:
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi John,
>
> Having used both systems, I know EXACTLY what you mean.  I have not
> figured a way to use ROI scanning on the Leica as it is on the Zeiss.
> For those of you not understanding the Zeiss system, it allows the user
> to define a ROI to scan using a rectangle, oval, or draw-your-own.  I
> used that function, for instance, to collect a stack of images of one
> cell in a field.  I have not found any way to do the same with the
> Leica--only ROI for analysis.
>
> J
>
> andrea manazza wrote:
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Hi John. I'm not quite sure I understood your question; anyhow, if I get
> > the point, the ROI on the SP2 might be used both for analysis and for
> > scanning (selective bleaching for FRAP, eg).
> >
> > I hope this is what you were looking for.
> >
> > Best wishes.
> >
> > Andrew
> >
> >
> >
> >
> >
> >
> >
> >
> > With the Zeiss confocal systems, it is posible to scan only within a
> > Region Of Interest.  Is this posible with the Leica SP2 software or is
> > the Leica ROI only intended for analysis?
> >
> > Thank you.  John.
> >
> >
> >
> > dott. Andrea Manazza
> > Dipartimento di Oncologia, Università di Torino
> > CeRMS, ospedale Molinette
> > Via Santena, 7 - 10126 Torino, Italia
> > lab: +39-11-6336859
> > fax: +39-11-6336887
> >

--
Russell McConnell
Manager, Tufts-NEMC Imaging Facility
Department of Neuroscience
http://www.neurosci.tufts.edu/Imaging