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Dear John,

It is possible to do this, but you may not have the buttons in your control
panel on the working screen (the laser controls are at the bottom of our
screen, which is where our ROI controls are).  I think you need to customise
your tools - the button is in one of the Tools menus and you need to open
this and drag the button to the controls at the bottom of the screen.
There's another button for setting up excitation wavelengths, etc. too.
cheers,
Rosemary



> From: Jill Pflugheber <[log in to unmask]>
> Reply-To: Confocal Microscopy List <[log in to unmask]>
> Date: Wed, 31 Mar 2004 12:47:01 -0500
> To: [log in to unmask]
> Subject: R: ROI scanning
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi John,
>
> Having used both systems, I know EXACTLY what you mean.  I have not
> figured a way to use ROI scanning on the Leica as it is on the Zeiss.
> For those of you not understanding the Zeiss system, it allows the user
> to define a ROI to scan using a rectangle, oval, or draw-your-own.  I
> used that function, for instance, to collect a stack of images of one
> cell in a field.  I have not found any way to do the same with the
> Leica--only ROI for analysis.
>
> J
>
> andrea manazza wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi John. I'm not quite sure I understood your question; anyhow, if I get
>> the point, the ROI on the SP2 might be used both for analysis and for
>> scanning (selective bleaching for FRAP, eg).
>>
>> I hope this is what you were looking for.
>>
>> Best wishes.
>>
>> Andrew
>>
>>
>>
>>
>>
>>
>>
>>
>> With the Zeiss confocal systems, it is posible to scan only within a
>> Region Of Interest.  Is this posible with the Leica SP2 software or is
>> the Leica ROI only intended for analysis?
>>
>> Thank you.  John.
>>
>>
>>
>> dott. Andrea Manazza
>> Dipartimento di Oncologia, Universit