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March 2004

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From:
ray hester <[log in to unmask]>
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Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 10 Mar 2004 09:54:23 -0600
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Does anyone know if you can lyse human red blood cells while leaving retics
intact?

" All samples were run in the resistant RBC mode to obtain complete
erythrocyte lysis. "

The above sentence in the article found at the following link,

http://www.laboratoryhematology.com/stories/storyReader$248

seems to hint at this but I wasn't sure.  If it can be done, can someone
tell us what procedure to use?  The only human rbc lysis reagent I'm
familiar with is buffered ammonium chloride (haven't done it in awhile, but
I think that's what we used to use).  Thanks for any information and
suggestions.

Ray Hester
Univ. of South Alabama
Mobile, AL
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