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You could also try Matrigel from BD.

http://www.bdbiosciences.com/discovery_labware/Products/cell_environments_and_ECMs/extracellular_matrix/basement_membrane_matrix/

Mike


>===== Original Message From [log in to unmask] =====
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>I use poly-L-lysine hydrobromide from Sigma-Adrich (product number
>P1274). This has a molecular weight range of 70,000 to 150,000. Poly-L
>or Poly-D lysine is fine, and a variety of grades will work. If you are
>worried about affects on your cells (particularly if long term
>culturing) then you may like to use the grade that is tissue culture
>tested. However, serum in the media will usually result in the cells
>lifting after a while.
>
>
>
>You can also buy the poly-L-lysine as a cell culture tested 0.01%
>solution (P4707), or with added preservative (P8920). This is convenient
>and works well.
>
>
>
>There are also many other cell adhesives that may be better than
>poly-Lysine. Others may like to comment on this.
>
>
>
>Hope this helps,
>
>
>
>Regards, Alan Hibbs.
>
>BIOCON (publishers of the manual "Confocal Microscopy for Biologists",
>HYPERLINK "mailto:[log in to unmask]"mailto:[log in to unmask])
>7 Walhalla Drive                 Phone: 61 3 9876 9822    FAX: 61 3 8660
>2290
>Ringwood East VIC 3135        Director: Dr. Alan R. Hibbs
>Australia
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[log in to unmask]] On
>Behalf Of Coetzee, Mr S. H Physics Science
>Sent: Tuesday, 6 April 2004 11:44 PM
>To: [log in to unmask]
>Subject: Poly-D-lysine hydrobromide
>
>
>
>Dear All
>
>Which grade of Poly-D-lysine hydrobromide is used to encourage cells to
>stay on glass slides?
>
>Since my UB mail address is not reliable please send
>
>a copy of all urgent mail to HYPERLINK
>"mailto:[log in to unmask]"[log in to unmask]
>
>Mr S. H. Coetzee
>Electron Microscope Unit
>University of Botswana
>Private Bag 0022
>Gaborone
>Botswana
>
>Phone     : +267 355 2462/5222
>Mobile    : +267 714 55701 (Now active)
>
>Fax       : +267 318 5097
>e-mail    : [log in to unmask]
>
>
>
>
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>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
Michael C. Adams
Microscopy & Imaging Manager for Dr. Clare Waterman-Storer
Laboratory of Cell Motility Studies
Department of Cell Biology
The Scripps Research Institute
Attn: Mail Code CB-163
10550 North Torrey Pines Road
La Jolla, CA 92037

TEL 858.784.9244
FAX 858.784.7521
EMAIL [log in to unmask]
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>