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Dear all,

Lately I have been testing different commercially available software
packages to quantify colocalization in confocal images. So far I have tested
Colocalizer Pro for MacOS X, the colocalization module of Bio-Rad's
Lasersharp 2000, and the Fluorescence CV plugin of IPlab. When I analyze the
same image with all three packages, I get very different values for the same
parameter. For instance, Pearson's correlation coëfficient can vary between
0.13 and 0.81 depending on which program performs the math.

Does anyone else have similar findings? I would really like to know which
program I can rely on.

Thanks in advance for input.

Jack

-- 
Jack A. Valentijn, Ph.D.
Asst. Professor, Dept. of Biochemistry and Cell Biology
Director, Center for Cell Imaging
Faculty of Veterinary Medicine
University of Utrecht
Yalelaan 2
3584 CM Utrecht
The Netherlands

Voice: +31 30 253 4308
Fax: +31 30 253 5492