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Fluorescently labeled G actin may be introduced into cells via
microinjection or, maybe, by some other method such as sonication or
electroporation.  This will be incorporated into f-actin at the filament
ends.  ANother option is to tensfect for GFP tagged actin.
-mc

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On Mon, 31 May 2004, Louis Villeneuve wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Bonjour,
> 
> I want to stain F-Actin in living myo-fibroblast (in culture).  what would
> be the reagent of choice?  Of course, I want it to be non toxic and membrane
> permeable.
> 
> Any ideas?
> 
> Thanks for the answers!!!
> 
> Louis
> Louis R. Villeneuve
> Assistant de recherche - microscope  confocal
> Institut de Cardiologie de Montréal- Centre de Recherche
> 5000 est Bélanger, Montréal
> Québec , Canada
> H1T 1C8
> 
> 514-376-3330 ext3511
> 514-376-1355 (Fax)
> 
>