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Hi Mao.  I don't believe that size matters, except for the thickness of
course.  Most optics are corrected for 0.17mm coverslip thickness.  

Cheers!
Mike
 

-----Original Message-----
From: mao [mailto:[log in to unmask]] 
Sent: Friday, July 09, 2004 11:22 AM
To: [log in to unmask]
Subject: Is there a lower-limit to the size of coverslip

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Hi there,
I am a novice to confocal and have a ignorant question. I do live tissue
GFP
imaging and use a tiny piece of glass coverslip(about 0.3mm x 0.5mm) to
cover
my sample because larger sizes would be inconvenient. The images look OK
but I
am not sure if this is theoretically valid. Can I use a coverslip as
small as
I want, or does it have to be larger than a certain size in order for
the
optics to work properly?
Thank you very much,
Mei