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An additional course has now been set up for April 2005, for details,
please see below.

CONFOCAL MICROSCOPY
4-Day Intensive Hands-On Course

Website: http://www.york.ac.uk/biology/tf/

Basics, Live-cell imaging, FRET, FRAP and
Spectral Unmixing

12th-15th April 2005
Technology Facility, Dept. Biology
University of York, UK

Instructors will include

Peter O'Toole, University of York
Les Borland, Carl Zeiss
Andrew Hubbard, Perkin Elmer
and special guest lecture.

Tutorials and practical sessions will cover both basic and advanced
confocal microscopy. The course will enable participants to realise the
potential behind many developing confocal techniques and realise the
simplicity of applying the techniques to a broad range  of sample types
and fluorescent labels. Both novice and more experienced users welcome.

Preliminary Programme
DAY AIMS
Day 1: Basics, Familiarisation, x,y,z and multicolour imaging.
Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on

laser scanning confocal.
Day 3: Advanced Techniques covering spectral unmixing and FRET with
fluorescent proteins.
Day 4: Further advances. More samples to explore confocal limits, and
time for participants own samples.

Thursday evening will include a special guest speaker and course meal.

The course will utilise many different sample types ranging from
cultured monolayers to plant cells. A diverse range of fluorescent
labels will also
be used, which will include various fluorescent proteins (CFP, GFP and
YFP) and classic antibody labels. Over the four days, three confocal
microscopes (2x Zeiss LSM 510 meta scan heads on both an invert and
upright microscope, PerkinElmer UltraVIEW) will be utilised.

Accommodation (en-suite), breakfast and lunches will be provided for the

length of the course.  Evening meals will be provided on both Tuesday
and Thursday evenings.
Places are limited to 8 participants to permit full hands-on practice,
so please book early to avoid disappointment.
For further information, please visit the course website at:
http://www.york.ac.uk/biology/tf/
 or contact Margaret Newton 01904 328821, [log in to unmask]



--
Dr Peter O'Toole
Imaging and Cytometry Laboratory Manager
Technology Facility
Department of Biology (Area 15)
University of York
PO Box 373
YORK
YO10 5YW

Tel : +44 (0)1904 328722
Fax : +44 (0)1904 328804
email : [log in to unmask]
www.york.ac.uk/biology/tf