Search the CONFOCAL archive at
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Hi James,
Thanks for the reply.  I would be interested in your presentation if it
isn't too much hassle to find it.  I feel that I finally have the knowledge
to develop PSF's for the objectives on our system and thought it would be
good to see what others have collected.  I'll be assessing a PCM2000
confocal on a Nikon E800 using several different oil immersion objectives
and hopefully a water immersion lens.
Thanks again,
Carl


Carl Boswell, Ph.D.
Dept. of Molecular and Cellular Biology
Univ. of Arizona
520-626-8469
520-621-3709 FAX
----- Original Message -----
From: "James Beals" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Monday, August 23, 2004 6:47 AM
Subject: Re: PSF examples?


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello Carl,
> I have a new position at the university, and shut down that server.
> About finishing the project...
> Well the student was awarded her Ph.D and so that part of the project
> was completed.
> She is a software developer, working on deconvolution.
> Her software relied on an known and measured PSF. Rather inconvenient
> due to differing convolution within different tissues.......but just
> good enough to get the Ph.D.
>
> I did not complete the project (collecting data from all objectives)
> just the 63x 1.2NA objective with 488nm laser.  Because the scope is in
> a core facility, I could not address some of the issues brought up.
> like the asymmetry above and below the microsphere.
> This was only apparent with the pinhole all the way open.  I have the
> images some were...I will dig them up if you are still interested. I
> have them in a presentation archived.
>
> James Beals
> Research Assoc. II
> University of Michigan
> Mental Health Research Institute
> 205 Zina Pitcher Place
> Ann Arbor, Mi 48109-0720
> [log in to unmask]
>
>
> On Wednesday, August 18, 2004, at 12:53  PM, Carl Boswell wrote:
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Hi James,
> > I'm trying to define the PSF's for our Nikon confocal and came across
> > this
> > communication of yours from a year ago.  Do you  still have the image
> > data
> > available online?  The link below doesn't work for me.  Did you finish
> > the
> > project?
> > thanks,
> > Carl
> >
> >
> > Carl Boswell, Ph.D.
> > Dept. of Molecular and Cellular Biology
> > Univ. of Arizona
> > 520-626-8469
> > 520-621-3709 FAX