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Why not use a glass bottom multi-well plate. A 96 well plate requires
as little as 50-60 ul to cover the bottom. Since you are looking from
the bottom a meniscus is not a problem.
-Neal
Neal R Gliksman, Ph.D.
Senior Application Scientist
Marketing Department
Molecular Devices Corporation
402 Boot Road, Downingtown PA 19335
http://WWW.Universal-Imaging.Com
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Guenter Giese
Sent: Tuesday, November 30, 2004 12:59 PM
To: [log in to unmask]
Subject: Cover glass bottom culture chambers (small volume wells)
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Hi all,
I am looking for cover glass #1.5 or #1 bottom culture chambers which
allow growth of cells in small volumes (< 200 microliters ) of culture
medium and scanning on an inverted microscope.
I already know ( and use) the LabTek 8-well cover glass bottom chambers,
but any multiwell chamber with more / smaller wells would be
advantageous for the following reasons:
- application of precious agents in smaller volumes
- rapid scanning / irradiation of all cells in a well (using a low
magnification objective)
- multiple samples side by side
------------------------------------------
Dr. Guenter Giese
Light Microscopy Facility
Dept. of Biomedical Optics, MPI fuer Medizinische Forschung Jahnstr. 29,
D-69120 Heidelberg, Germany Phone (+49) 6221-486-360 (Fax: -325)
e-mail: [log in to unmask]http://www.mpimf-heidelberg.mpg.de/~ggiese/lightmicro
