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Silicone rubber works well also but make sure you use Dow 3140 it
applies the same as silicone grease, cures at room temperature and is
cell friendly.

Regards, Michael
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Morrison, Ian E
Sent: Tuesday, November 23, 2004 7:17 AM
To: [log in to unmask]
Subject: Re: sealing of immunofluorescent slides

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Try using a 5ml plastic syringe with a 200ul micropipette tip (yellow)
on the end - you may need to adjust the end of the tip with a scalpel to
get the correct aperture.

We use these filled with silicone grease (a type of vacuum grease) to
lay a fine line of sealant around the edge of the coverslip, about 2mm
in from the edge; the soft silicone can be compressed to create the
seal. But BEWARE of getting any grease on the objective lens as it
requires  serious solvents to remove.  Ian 

Dr. I.E.G.Morrison       [log in to unmask]
Dept.Biological Sciences, University of Essex
Wivenhoe Park, Colchester, Essex CO4 3SQ
Tel: 01206-872246           Fax: 01206-872592----------------------



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]On
Behalf Of Mary Ellen Pease
Sent: Tuesday, November 23, 2004 2:52 PM
To: [log in to unmask]
Subject: sealing of immunofluorescent slides


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I've heard varying opinions regarding the use of sealants for slides
mounted in fluorescence friendly media such as Vectashield.  We are
looking at eGFP cells with immuno label for cell type markers visualize
with either Cy3 or Cy5.  Is it true that nail polish is problematic for
eGFP preps and if rubber cement is a better sealing option, any
suggestions on how to apply it best? We've tried applicator sticks or
pastuer pipets with more mess than nail polish.  Any secrets that can be
shared?

Thanks!
Mary Ellen

Mary Ellen Pease, M.S.
Laboratory Manager
Glaucoma Research Lab & Microscopy and Imaging Core Facility
Wilmer Eye Institute, Johns Hopkins Hospital
175 Woods Research
600 N. Wolfe Street
Baltimore, MD 21287
410-955-3337 (phone/voicemail)
443-287-2711 (fax)
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