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Are the images shown in the paper raw or were they filtered in anyway
before calculating the ratios? It is not mentioned in the methods. That
could cause artefactual "spots"

Leoncio A. Vergara MD
Research Assistant Professor
Department of Neuroscience and Cell Biology
Director, Optical Imaging Laboratory (O.I.L.)
University of Texas Medical Branch
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Stephen Cody
Sent: Tuesday, December 07, 2004 7:20 PM
To: [log in to unmask]
Subject: Re: Better than possible confocal images?

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Dear Christof,

I'd have to share your scepticism. Considering FIG 1B in this paper is
an intensity map, and the dendrite that is highlighted in FIG IC can
hardly be seen in Fig 1B I'm not convinced that what they show is
anything but noise or edge artefact.

Cheers

Stephen H. Cody

Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute For Cancer Research
PO Box 2008 Royal Melbourne Hospital
Parkville  Victoria    3050
Australia
Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
email: [log in to unmask] 
www.ludwig.edu.au/labs/confocal.html
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