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I would add incubation with trypsin and/or pepsin to remove all residual
proteins and a strong detergent.
We may have to begin reusing the special Olympus TIRF coverslips.  One
idea we have (but havene't tried yet) is commercial laundry detergents
which are enzyme detergent cocktails.
 -Michael

> too much of a problem. The hard parts are washing to the point of
> scrupulous cleanliness and sterilizing. One approach would be to wash
> the culture vessels with soap/water, rinse well, soak in 70% ethanol and