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December 2004

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Subject:
Excitation spot size
From:
David Knecht <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 16 Dec 2004 13:39:47 -0500
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Is the excitation spot size for a laser scanning confocal microscope
the same as that of an epifluorescence widefield microscope (same
objective, etc.)?  Put another way, if I removed the PMT pinhole,
would I get an image comparable to a widefield image?  Thanks- Dave
--

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.   U-3125
Storrs, CT 06269-3125
[log in to unmask]
860-486-2200      860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html

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