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Dear Benny,
We have been using Fluoromount G to mount coverslips on thick  
fluorescent samples, such as 40 µm vibratome sections.  It is water  
soluble, we only wick excess moisture from around the sections so that  
they will stick to the slides just enough to prevent them from moving  
around when the coverslip applied.  I suppose that you could use it  
without coverslip,  if you are using objectives designed specifically  
to work without coverslips.  Otherwise lack of a coverslip will create  
huge problems with spherical aberration, since most objectives are  
designed with a requirement for a 0.17 mm thickness. The degree of  
aberration will vary with the type of objective and its NA.

Regards,
Glen

On Dec 30, 2004, at 9:57 AM, Xin, Xiangrong wrote:

> Search the CONFOCAL archive at  
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>  Dear all,
>   
> I am a new guy in confocal microscopy. Currently, I am working on 3D  
> reconstruction with confocal image sequence. 40um tissue section is  
> used. Can anyone recommend some hardening mounting media (with or  
> without DAPI) to me? It can harden completely and I will not use  
> coverslips.
>   
> I'd appreciate if anyone can share experience on that with me. Have a  
> good holiday to all!
>  
> Best regards
> Benny Xin
> Research Assistant
> Taylor's Lab
> Center for Cardiovascular Repair
> University of Minnesota
>  
>
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
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