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Tony, Glen, et. al.,

Thanks for the tips....the (hidden) compression box was the culprit. I'm
back in business.

Cheers,

Tom

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Tony Collins
Sent: Thursday, January 20, 2005 10:01 AM
To: [log in to unmask]
Subject: Re: ImageJ/Zeiss LSM file problem


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Hi Tom,

The LSM software will allow you to compress the LSM file as an option -
  it's hidden in the extreme bottom left of the in the Save dialog.

ImageJ currently does not support compressed tiffs, although I
understand this is in the process of being addressed.

You may need to re-save you LSM files with the compression "off". Once
turned off it seems to stay off. I guess it's off (or not implemented)
in your older LSMs...?

Regards,

Tony

Moninger, Thomas wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Groups,
>
> I have encountered a related problem with ImageJ..... I have access to
> several Zeiss 510's, three of them a few years old and one of them a
new
> NLO META. For some reason I can not open images from the newest system
> using the ImageJ LSM reader plug-in. Every time I try I get an
"Image/J
> does not support compressed TIFFs" error. It does not matter whether
the
> image is one, two or three channels or if a transmitted light image is
> also present. I made sure the  images are 8 bit.
>
> Any ideas?
>
> Thanks,
>
> Tom

--
Tony Collins, Ph.D.
Facility Manager
Wright Cell Imaging Facility
Toronto Western Research Institute
13-407 McLaughlin Pavilion
399 Bathurst Street
Toronto, ON. M5T 2S8
tel. (416) 603 5367 fax: (416) 603 5745
http://www.uhnresearch.ca/wcif