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Hi all,
when I need to convert our LSM 410 confocal microscope into an upright
microscope I use a "microscope inverter" sold by LSM technologies Inc. With
this device, I can easily use a water dipping objective coming from above
the specimen. This device work as well on invert and on upright microscope
and is a lot cheaper than a microscope.
Of course I don't have any financial interest in LSM technologies..
Robert
>Robert Monette, Ph.D.
>
>Multi-Photon & Confocal Imaging facility
Neurophysiology Group
>Institute for Biological Sciences
>National Research Council of Canada
>Building M-54, Montreal Road
>Ottawa, Ontario, Canada K1A 0R6
>tel: (613) 991-6752 fax: (613) 941-4475
>[log in to unmask]
>
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of J. Scott Gens
Sent: Wednesday, January 19, 2005 2:27 PM
To: [log in to unmask]
Subject: Re: "special" technique
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Bob-
Looks like I read your original post a little too hastily. I was thinking
more along the lines of protecting non-dipping lenses on inverted scopes
from fluid leaks- particularly sealing the gap between the objective and its
correction collar.
Your reply makes clear that you have a different problem in mind altogether.
So basically your colleagues wish to flip your setup over to use a dippping
lens on an inverted platform and use the product to provide a water layer
that will move as the lens focuses up and down through a 3D stack?
I'm also having a hard time visualizing how that would work, sure would be
an interesting trick, and I'd also like to hear a solution if someone on the
list knows one.
J. Scott Gens
Quoting "Robert J. Palmer Jr." <[log in to unmask]>:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Thanks for the reply! I'm a little confused as to how exactly this
> works regardless of the "product" involved. In our particular case,
> we have a very irregular opaque substratum on which our biology takes
> place. We can image this nicely using a dipping lens on an upright,
> but some colleagues of ours wish to do the same thing and have only an
> invert. We can get the substratum attached to a slide with the butter
> side down (protruding through the lens cutout in the microscope stage,
> but we then must get a water film, actually about 2 mm of water,
> between the substratum and the lens. Furthermore, this water meniscus
> must accommodate the motion of the stage during confocal sectioning.
> I simply have a mental block when I try to imagine how your glove
> finger would be attached to the lens to form a pool of water in which
> the lens would operate. Doesn't the water overflow when the stage is
> moved during sectioning? Doesn't the glove touch the stage at some
> point? Do you have any pictures of this setup?
> Thanks for you advice!
> By the way, another researcher here saw my post and also wants to
> know what I learn. Maybe we can give you a "personal communication"
> in our next publications :) :)
> Rob Palmer
>
> >Search the CONFOCAL archive at
> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >Not sure where I first heard about the idea you're describing, Bob,
> >but
> it's
> >probably been floating around for a while.
> >
> >A cheaper and less embarassing product more readily found product in
> >a lab environment ( no need for a trip to the corner drug store),
> >I've found that cutting one finger off a latex lab glove and slipping
> >it over the lens will serve much the same purpose in protecting
> >objective lens from liquids.
> >
> >Trial and error quickly establishes what size of glove and correct
> >finger
> or
> >thumb fits a given objective.
> >
> >Only caveat is the latex seemed to break down fairly rapidly, so
> >inspection for small holes or wear prior to use coupled with regular
> >replacement would
> be
> >advisable.
> >
> >J. Scott Gens, PhD
> >Post-doctoral Fellow
> >Indiana University School of Medicine
> >Division of Nephrology
> >
> >Quoting "Robert J. Palmer Jr." <[log in to unmask]>:
> >
> >> Search the CONFOCAL archive at
> >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>
> >> Hi all - I remember a post from a confocaler "down under" who had
> >> developed an approach to using a water-immersible (dipping lens, no
> >> coverslip) on an inverted platform. The approach involved a
> >> prophylactic.... Can anyone put me on to this approach in the
> >> literature or put me in contact with the person who advocated it?
> >> I would also be interested in other approaches to achieve this
> >> end. Thanks!
> >> Rob Palmer
> >> --
> >> Robert J. Palmer Jr., Ph.D.
> >> Natl Inst Dental Craniofacial Res - Natl Insts Health
> >> Oral Infection and Immunity Branch
> >> Bldg 30, Room 310
> >> 30 Convent Drive
> >> Bethesda MD 20892
> >> ph 301-594-0025
> >> fax 301-402-0396
> >>
> >>
> >>
>
>
> --
> Robert J. Palmer Jr., Ph.D.
> Natl Inst Dental Craniofacial Res - Natl Insts Health
> Oral Infection and Immunity Branch
> Bldg 30, Room 310
> 30 Convent Drive
> Bethesda MD 20892
> ph 301-594-0025
> fax 301-402-0396
>
>
>
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