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Hi,

If you want to calibrate your system, there is an article on calibration
of confocal systems by Lerner et al.:

Lerner JM, Zucker RM.
Calibration and validation of confocal spectral imaging systems
Cytometry. 2004 Nov; 62A(1):8-34.

Regards,

Peter

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Peter Van Osta, MD

Director Imaging
MAIA SCIENTIFIC
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-----Oorspronkelijk bericht-----
Van: Confocal Microscopy List [mailto:[log in to unmask]]
Namens
Mary Ellen Pease
Verzonden: donderdag 27 januari 2005 14:45
Aan: [log in to unmask]
Onderwerp: Units of Zeiss Meta Emission Intensity Graphs?

Search the CONFOCAL archive at
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Hi all,

I have spent a couple hours "google"ing and reviewing manuals/pdfs provided
by Zeiss without success to locate the following:

What are the units for the emission intensity graphs (and tables) one can
create when selecting an ROI within a  lambda stack?  When the graph data
are not normalized, the Y axis for intensity appears to read from 0-250
(with an 8 bit image - I've not taken any 12 bit yet), yet no unit is given.
Does this mean that it is reading brightness on a scale of
0-256 like an 8 bit digital cameras would and if so, what is the proper
technical label for this attribute?

Thanks all.

Mary Ellen Pease, M.S.
Laboratory Manager
Glaucoma Research Lab & Microscopy and Imaging Core Facility Wilmer Eye
Institute, Johns Hopkins Hospital
175 Woods Research
600 N. Wolfe Street
Baltimore, MD 21287
410-955-3337 (phone/voicemail)
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