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I usually perform lambda stacks exciting with 750nm and the range is 380 to
720.
Dardo Ferrara
Emory University
>From: Badri Roysam <[log in to unmask]>
>Reply-To: Confocal Microscopy List <[log in to unmask]>
>To: [log in to unmask]
>Subject: Re: Zeiss META Spectral Range
>Date: Tue, 4 Jan 2005 09:18:05 -0500
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>While we're on this topic, I have a related question: How many distinct
>fluors, and what types of molecular markers (genomic, proteomic, ionic,
>others?) are people able to image using this system? Are there interesting
>multiple-label / multi-dimensional image analysis problems that people are
>encountering?
>
>Wishing everyone a productive and happy 2005.
>
>Badri Roysam
>Professor, Department of Electrical, Computer and Systems Engineering
>Associate Director, NSF Center for Subsurface Sensing & Imaging Systems
>(CenSSIS ERC)
>Rensselaer Polytechnic Institute
>110 8th Street, Troy, New York 12180-3590.
>Office(JEC 7010): 518-276-8067, Lab(JEC 6308): 518-276-8207, Fax:
>518-276-8715
>Email: [log in to unmask], Web: http://www.rpi.edu/~roysab
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > There are more ranges available depending on the lasers in the system.
> > The 411.3-753.7 range is for a system with a 405 laser. Systems with a
> > 458 line as lowest line have a range from 456-798. Probably the angle of
> > the grating is just shifted a bit to change the range. The range can not
> > be changed by the users.
> >
> > Hope this clarifies a bit, Gert
> >
> > --
> > wigGert van Cappellen
> > mailto:[log in to unmask]
> > Tel. +31-10-40 87578; FAX +31-10-40 89461
> > applied Optical Imaging Centre
> > http://www.eurasmusmc.nl/oic/
> > Reproduction and Development
> > Erasmus MC, Dr. Molenwaterplein 50, 3015 GE ROTTERDAM
> > The Netherlands
> >
> >
> >
> > simon walker (BI) wrote:
> >
> > >Search the CONFOCAL archive at
> > >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> > >
> > >The META detector covers a spectral range of 411.3 nm to 753.7 nm. You
> > >can select the range of wavelengths detected, but because the META is
>an
> > >array of 32 PMTs each covering 10.7 nm bandwidth, you are restriced to
> > >10.7 nm increments when adjusting your range.
> > >
> > >You can 'bin' any number of the 32 pmts into multiple channels, or you
> > >can use the META in lambda mode where you select you detection range
>and
> > >get a read out from each individual pmt.
> > >
> > >Hope that's of use.
> > >Simon
> > >
> > >
> > >
> > >
> > >-----Original Message-----
> > >From: Confocal Microscopy List [mailto:[log in to unmask]]
>On
> > >Behalf Of Stamatis Pagakis
> > >Sent: 04 January 2005 11:04
> > >To: [log in to unmask]
> > >Subject: Zeiss META Spectral Range
> > >
> > >
> > >Search the CONFOCAL archive at
> > >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> > >
> > >Hello and HAPPY NEW YEAR
> > >
> > >could someone please inform me which is the spectral range of the ZEISS
> > >META detector? I could not get a figure from the ZEISS website and the
> > >list archives have 2 different figures:
> > >
> > >460-800nm and 380-720nm
> > >
> > >Is META tunable in terms of choosing the range?
> > >
> > >Thank you
> > >
> > >
> > >***************************************
> > >Dr. Stamatis Pagakis
> > >Confocal microscopy and Image Analysis Lab
> > >National Institute for Medical Research
> > >Mill Hill, London NW7 1AA
> > >Tel: +44 (0)20 8816 2621
> > >Fax: 020 8906 4477
> > >Mob: 07900 988 150
> > >
> > >
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