Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

For Mitotracker Orange or for TRITC a 543 line would be better...

Mike

----- Original Message -----
From: Aryeh Weiss <[log in to unmask]>
Date: Thursday, May 12, 2005 3:31 pm
Subject: Re: 543 vs 561 lasers

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Mcnamara, George wrote:
> >
> > If there are ANY dyes that excites optimally at 543 (vs 561)
> that do not
> > excite at 514 I would like to hear about them.
> >
> > I think that 488/514 ... 561 ... 633(or 647) is better spacing
> than 514 ...
> > 594 ... 647 for biologically useful dyes.
> >
>
> I agree that the 561 DPSS laser is better than the 543nm HeNe,
> especially if you
> will want to do acceptor photobleaching. It will also excite Cy3
> quite well,
> even though in principle it is about 15nm longer than the listed
> excitationpeak. However, I would advise you to get the 594 HeNe
> also. It will excite TR
> better than the 633nm. The 561nm will also excite TR, but you may
> want to excite
> TR without exciting Cy3.
>
> You will find that you can excite dyes well off of their
> excitation peaks.
> The main reason for having a lot of excitation lines is to enable
> selectiveexcitation of longer wavelength dyes without suffering
> bleedthrough from the
> shorter wavelength dyes.
>
> --aryeh
> --
> Aryeh Weiss
> School of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
>
> Ph:  972-3-5317638
> FAX: 972-3-5340697
>