Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Ray,

Contact Dr. Vitaly Vodyanoy at Auburn.  He has been doing a lot of work
with bacteria and may have a solution.  I've included his email above.

Thanks
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to
learn more about "Optimizing LIght Microscopy".  Copies still available
through MME... even for class-room lots ... and we give quantity discounts.
Call us at (972)954-8011.
At 09:55 AM 6/20/2005, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi,
>
>Does anyone have suggestions for detecting size changes in bacteria, in
>particular Vibrio vulnificus, reported to be
>0.5 to 0.8 micron in diameter and 1.4 to 2.6 microns in length.  I know
>it would only be qualitative at best, but can such measurments be done
>by flow?
>
>I don't have any idea what the possibilities for size increase might be
>in the above case - microns or tenths of a micron - the latter I
>suspect.  If tenths, flow cytometry wouldn't be the answer, but maybe
>microns?  If not flow, what _would_ be the definitive method of
>determining bacterial size change?   EM of some type?
>
>Thanks for any info.
>
>Ray Hester
>Univ. of South Alabama