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HI, Michal-
I don't know whether you got the advise you were seeking long ago. I
believe most people would approach this problem by use of freezing
methods. Though, for some reasons, such as the tiny size of your
samples, that may not be the more desirable for you.
I noted as I went to delete this message, that you are working on
stem cells. We have a universal medium, of which the composition is
unpublished, that I feel is appropriate for stem cell growth. We are
going to market it soon (by September approximately). Would the
people you are collaborating with like to have a sample to try it out?
Carol
>Due to all sort of difficulties I am not sure anymore if this reached the
>forum. My apology if it is a re-posting.
>
>
>Dear All,
>
>While my question is only partly related to fluorescence/confocal/microscopy
>I feel that this might be a right forum to pose it.
>
>
>We require a procedure that will reliably provide good morphology and
>immunocytochemistry (preferably fluorescence detection-based) of embryoid
>bodies differentiating from embryonic stem cells. In our hands, embryoid
>bodies reach up to 1 mm in diameter and, as expected, comprise several
>tissue types.
>Ian Gibbins has been kind enough some time ago (August 14, 2000) to describe
>a polyethylene glycol (1,000 MW / 1,450 MW) embedding method together with
>fixation procedures they are using for neural tissue.
>
>In addition to polyethylene glycol, several people have been using polyvinyl
>alcohols. I would love to receive comments regarding applicability of
>either medium to sectioning and immunolocalization.
>
>Finally, products such as ImmunoBed (Polysciences) and Technovit 8100
>(Heraeus Kulzer) have been also advertised as extremely suitable for the
>aforementioned tasks.
>
>Predictably, a novice such as myself, is getting utterly confused trying to
>sort out what should be tried first and what not at all.
>
>Any practical advice shall be highly appreciated.
>My apology to all of you who are not interested.
>
>
>
> Dr. Michal Opas
> Cell Biology
> University of Toronto
> 1 King's College Circle
> Medical Sciences Building, rm. 6326
> Toronto, Ontario, M5S 1A8 Canada
>--------------
> phone: (416) 978-8947 (laboratory)
> (416) 971-2140 (office)
> fax: (416) 978-3954
> e-mail: [log in to unmask]
>www homepage: http://www.utoronto.ca/anatomy/opas/start.htm
--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: [log in to unmask]
http://www.bgsu.edu/departments/biology/facilities/MnM
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