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A caution against using "Super Glue" or any other cyanomethacrylate
containing product anywhere near optical surfaces!  Cyanomethacrylate
outgases as it cures or dries.  While commercially available coverslip
mounting media like Permount consist of a resin dissolved in a solvent,
cyanomethacrylate is a liquid.  What you smell when "Super Glue" cures
is the same cyanomethacrylate that forms the bond.  This outgassed
cyanomethacrylate coats and damages nearby optical surfaces.  It is
difficult to remove cyanomethacrylate residue without damaging coatings.

Jeffrey Larson
Product Manager, Confocal Systems
Nikon Instruments Inc.
1300 Walt Whitman Road
Melville, NY 11747
(631) 547-8540


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Guy Cox
Sent: Tuesday, August 16, 2005 5:32 PM
To: [log in to unmask]
Subject: Re: Reflected light

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George Macnamara wrote:
> p.s. if you do not have a reflection slide, you can try to get the
Leica
> service engineer to come over with their's, or buy one for a few
dollars
> at
> http://www.nanofilm.com <http://www.nanofilm.com>

Or you can get one for virtually nothing by getting your
local SEM facility to sputter coat a #1.5 coverslip.
Superglue it to a slide (coating down) and you have
a permanent and invaluable test specimen.

                                        Guy

--
Associate Professor Guy Cox
Electron Microscope Unit,
University of Sydney,
NSW 2006, Australia

Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
http://www.guycox.net