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August 2005

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From:
Aryeh Weiss <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 18 Aug 2005 20:48:05 +0200
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Ian Dobbie wrote:

>
> The scattering is unlikely to be caused by the META system if it is
> visible in the other channels, and the dichroics for the other detectors
> are before the start of the META optics, and so should be unaffected
> by them. Sounds like there is something scattering either in the
> microscope, or in the scan optics. Can you get a good image down the
> eyepieces? If so it would seem to be in the scan head, if not, it is
> something in the microscope itself.
>

The widefield image is fine. Since the scattering is observed even when the meta
detector is not receiving light (100% mirror reflecting to the conventional
PMTs), it is probably not due to that system. However, the scatter really
reduces the usefulness of the meta detector.

--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-5340697

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