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Michael,
Try a incubating your sections in an aqueous solution of 0.01% Toluidine
Blue for 1-3 minutes post antibody labeling.

Combine
    10 mg toluidine blue
    100 mg sodium borate (you can omit this)
    100 ml dH2O
2. Stir until dissolved (about 30 min).
3. Filter just before use (I use a 0.2µm syringe filter), even if it's been
pre-filtered for storage.

Cheers,
Mark

-- 
Mark A. Sanders
Program Director, Imaging Center
College of Biological Sciences
123 Snyder Hall
1475 Gortner Avenue
University of Minnesota
St. Paul, MN  55108
Phone: 612-624-3454 | Fax: 612-624-1799
www.cbs.umn.edu/ic
Biodale Member 
On 9/7/05 10:22 AM, "Michael Model" <[log in to unmask]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Dear microscopists -
> 
> One of our users is working with formalin-fixed mouse testis tissue.
> We are seing strong autofluorescence from interstitial cells of
> Leydig. Does anyone know how to reduce it? Thanks in advance,
> 
> Michael Model
> Confocal Imaging Core,
> School of Biomedical Sciences,
> Kent State University,
> Kent, OH 44242
> tel. 330-672-2874
>