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The colocaliziation of the UV excitation and visible excitation  may not
be parfocal. Not all confocal microscopes make the necessary adjustments
to deliver colocalization
This should be definitely evaluated as it is critical for many
experiments.
Best wishes
bob

Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory
Reproductive Toxicology Division, MD 72
Research Triangle Park, North Carolina, 27711
Tel: 919-541-1585; fax 919-541-4017
e-mail: [log in to unmask]



                                                                        
             Perveen Biln                                               
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             RV.BUFFALO.EDU>                                    Subject 
                                      purchasing an new scope           
                                                                        
             09/23/2005 01:31                                           
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Hello,

We are looking to purchase a new microscope for live cell imaging and I
was hoping to get some feedback regarding the general advantages and
disadvantages of the different options.  Currently, we can choose
between a confocal such (as the zeiss meta), a spinning disk or a
two-photon.  One the main concerns against purchasing a confocal is that

many of the researchers would like use UV light, but are concerned about

the cost of purchasing and maintaining a UV laser.  Comments regarding
the most cost-effective scope and on the advantages / disadvantages of a

UV laser would be appreciated.

thank you!

--
Perveen Biln, M.Sc.
Microscopy and Imaging Consultant
Institute for Health Research and Education
Phone:             604.268.6807
Email:             [log in to unmask]