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Date: | Wed, 23 Nov 2005 09:18:27 +0100 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
FYI, 0.17 +/-0.01mm corrected coverslips have been available as a standard
item for a long time, e.g. from Assistent (Hecht Glaswarenfabrik).
Beat
At 02:15 23.11.2005, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>G’day List,
>
>Good news. A new “standard thickness” of coverglass for confocal microscopy.
>
>No commercial interest or association with this company or product.
>
>One of those subjects that keeps cropping up is the thickness of
>coverslips for confocal microscopy, in particular for use with water
>immersion lenses. We all want 170um thick coverslips, however the
>manufacturers of the glass can’t make it at a set thickness. Instead they
>measure the thickness of the glass that they have made, then sort it. They
>sort it into the gradings as previously mentioned on this list by Dan Focht
>
> > Here is a chart of industry standard coverslip thickness
>
> > 00 = 0.060 - 0.08
>
> > #0 = 0.080 - 0.120
>
> > #1 = 0.130 - 0.170
>
> > #1.5 = 0.160 - 0.190
>
> > #2 = 0.170 - 0.250
>
> > #3 = 0.280 - 0.320
>
> > #4 = 0.380 - 0.420
>
> > #5 = 0.500 - 0.60 mm
>
>The trouble with these standards is that 170um is not in the middle of any
>of them. For example, I have a water immersion lens that has a correction
>collar on it that is adjustable from 150 – 180 um. If I buy #1 coverslips
>they may be less than 150um and outside the range of my lens. If I
>purchase #1.5’s they may be grater than 180um and again unusable.
>
>After discussing this issue with our local supplier and custom cutter of
>coverslips, they have negotiated with the manufacturer of the glass a new
>grade of coverslip that will be of great benefit to us all. It is 0.170
>+/- 0.01mm.
>
>At the moment I only have one catalogue number for “25mm Circle”
>coverslips - CSC25(170)GP, but if you contact the supplier I’m sure most
>common shapes and sizes will be available. The supplier’s details are
>given below and they will fill orders from overseas. The pricing is reasonable.
>
>Address your enquiries to:
>
>Lomb Scientific Customer Service
>
>Lomb Scientific Pty. Ltd
>Head Office
>
>26 Atkinson Road
>(PO Box 2223)
>Taren Point NSW 2229
>
>Tel: 61-2 8536 6222
>Tel (2): 1300 725 973
>Fax: 61-2 8536 6250
>Toll Free Fax: 1300 30 30 25
>
>Email: <mailto:[log in to unmask]>[log in to unmask]
>
>http://www.lomb.com.au/
>
>Cheers
>
>Stephen H. Cody
>
>Microscopy Manager
>
>Central Resource for Advanced Microscopy
>Ludwig Institute For Cancer Research
>
>PO Box 2008 Royal Melbourne Hospital
>
>Parkville Victoria 3050
>
>Australia
>
>Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
>
>email: <mailto:[log in to unmask]>[log in to unmask]
>
>www.ludwig.edu.au/labs/confocal.html
>
><http://www.ludwig.edu.au/confocal>www.ludwig.edu.au/confocal
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