Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello,

I am attempting to characterize a viral vector.  Right now I am  
injecting a virus that makes soluble EGFP and collecting the liver,  
sectioning and taking photos of the native fluorescence.  I have a  
great signal but I am having a difficult time determining which cells  
are positive.  I would like to characterize which cells are  
transduced and get a general idea of the percentage transduced.  The  
easiest way to do this is probably DAPI staining I think, just to get  
an idea of the number of cells and possibly determine the percentage  
transduced.  Could someone recommend anything that will help me  
determine which cells are staining?  I am relatively new to  
microscopy especially fluorescence.  I would love something that I  
can visualize easily on a standard fluorescent microscope as well.   
Could I do an H&E staining on the sections and still see the  
fluorescent signal?  If not is there an alternative?  I would love to  
see a typical stain that would highlight the various cell types and  
then overlay my fluorescent signal in the same field.

Any and all advice is appreciated.

Sincerely,

Caroline Bass