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Hi Judy,
Try DRAQ5 from Biostatus in UK (http://www.biostatus.co.uk/index.htm).
Expensive but gives good results. In US, Axxora sells it:
http://www.axxora.com/?content=open.php%3FPID%3DBOS-889-001
Good luck,
- Damir
Judy Trogadis wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Fellow Confocalists
>
>I am looking for a nuclear counterstain for live cells which excites
>around 633nm. Molecular Probes' Syto dyes (59-64), while they do excite
>at that wavelength, also label mitochondria because of their positive
>charge.
>
>We are already staining mitochondria with a red/green apoptotic marker
>(red if live; green if apoptotic) so cannot change the wavelength of the
>mitochondrial marker to green - are there any?
>
>Thank you in advance for any suggestions
>Judy
>
>Judy Trogadis
>Bio-Imaging Coordinator
>St. Michael's Hospital, 7Queen
>30 Bond St.
>Toronto, ON M5B 1W8
>Canada
>ph: 416-864-6060 x6337
>pager: 416-685-9219
>fax: 416-864-6043
>[log in to unmask]
>
>
--
Damir Sudar - Staff Scientist
Lawrence Berkeley Laboratory / Life Sciences Division
One Cyclotron Road, MS 84-171, Berkeley, CA 94720, USA
T: 510/486-5346 - F: 510/486-5730 - E: [log in to unmask]
WWW: http://www.lbl.gov/lifesciences/labs/sudar_lab.html