Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Is this an Axiovert?  Does this appear with low intensity images?  It  
is likely that the Brewster window still in place below the objective  
turret and you are seeing some reflections from it.  I've not much  
experience on the LSM510, but have encountered these reflections with  
low intensity images.  You might try reduced laser power.  The window  
is very useful to prevent liquids from reaching sensitive parts in  
the scope base if the system is used for live cell imaging.

Regards,
Glen


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
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The box said "Requires Windows 95 or better", so I bought a Macintosh.
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On Feb 1, 2006, at 7:42 AM, Carlos Sanchez wrote:

> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi- 
> bin/wa?S1=confocal
> Dear list members,
>
>
>             We would appreciate a lot if you could help us with one  
> problem we observe from time to time in our Zeiss LSM510 Meta  
> system. We have tried many things (glass, samples, mounting medium,  
> objectives, etc.) without results. In fact, it is very difficult to  
> find the problem because it appears from time to time. It would be  
> ideal if you could take a look to some images and tell us if you  
> have found these strange patterns before, since you have a very  
> large experience in microscopy. Curiously, we had not seen these  
> effects before in the other two BioRad confocal systems of our  
> Facility.
>
>             You may download the images from: http://www.cbm.uam.es/ 
> confocal/pruebas/lsm510.zip (sorry but it is a big file)
>
>
> Thank you very much in advance fro your help.
>
> Yours sincerely,
>
>
>
> Carlos Sanchez Martin. PhD.
>
> Titulado Superior Especializado (C.S.I.C.)
>
> Servicio de Microscopia Optica y Confocal (S.M.O.C.)
>
> Centro de Biologia Molecular Severo Ochoa
>
> Universidad Autonoma de Madrid. Cantoblanco.
>
> 28049. Madrid. Spain.
>
> Tlf. 34-91 497 8049/34-91 497 7623
>
> Fax. 34-91 497 8087
>
> E-mail. [log in to unmask]
>
> Web S.M.O.C. http://www.cbm.uam.es/mkfactory.esdomain/webs/CBMSO/ 
> plt_Servicio.aspx?IdServicio=19
>
>
>