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We do it slightly differently. (there are always six ways to do 
anything in Photoshop!).

Remember to have a black background. Be in RGB mode. Save a copy of 
the original so you have all three colours to go back to. Go into the 
channels window. Select all and delete the colours you don't want.
Elaine

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi Griffin,
>
>I got it. Thank you very much. That save me a lot time. Thank you 
>again for your help.
>
>
>Jing
>
>
>On 1 Mar 06, at 4:34 PM, Perry, Griffin M wrote:
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Jing,
>>
>>The best way that we have found (using Version 3.0 Build 461) is to save
>>it as a .tiff.  Under .tiff, Save Options, Save as RGB Format.  Some
>>programs don't like the RAW Format.  I haven't saved and tested RAW
>>format with Adobe Photoshop, so it might be worth a try.  From here, you
>>label which colour for which channel (ours right now (488)Red 2,
>>(568)Green 1, (633)Blue 3, but you can change it to whatever you like).
>>Finish saving the .tiff.
>>
>>In Adobe Photoshop, there should be three different colours in the
>>picture.  If you want to isolate the three different channels (colours)
>>from one another, there are many ways in Adobe Photoshop CS (8.0).
>>
>>The way I isolate different channels is to make a copy of the original
>>image. With the copy selected, go to Image->Adjustment->Levels and pick
>>which channel you DON'T want (if you just want green select red).  On
>>the output, change them both to 0.  This will take out the red.  Next,
>>select blue and set output to 0.  In the copied image, you will only see
>>the green channel.
>>
>>Hope this helps,
>>
>>G
>>
>>
>>-----Original Message-----
>>From: Confocal Microscopy List [mailto:[log in to unmask]] On
>>Behalf Of JingHe
>>Sent: Wednesday, March 01, 2006 2:29 PM
>>To: [log in to unmask]
>>Subject: Nikon EZ-C1
>>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Hello,
>>I'm using Nikon EZ-C1 2.20 to get three different channel pictures. Is
>>there any way I could also open them in adobe photoshop in three
>>different color pictures? Thank you.
>>
>>Jing


-- 
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada (2003-2005)
University of British Columbia
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