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Dear list,

I am at the beginning of my "transition" from flow cytometry to 
confocal imaging (and I'm loving it!), and I have some basic questions.
We have a Zeiss LSM510 equipped with  488, 546, and 633 lasers.
I would like to use Qdots to avoid this horrendous bleaching problem. 
The questions are:
1. Can I use Qdots 700, or will the signal be too low (low energy far red)?
2. Can I use three different Qdots on the same sample and collect 
them from the same "line"? How about two? If their emission peaks are 
indeed so tight, maybe it will be possible?
3. Why don't people ever use APC (allophycocyanin) in microscopy, 
while we use it all the time in flow? The bleaching I see is 
comparable to that of Cy5, at least to my very unexperienced eye.

Thank you!

Giovanna



--------------------------------
Giovanna Borsellino, M.D., Ph.D.
Neuroimmunology Unit
European Brain Research Institute
Santa Lucia Foundation
Via del Fosso di Fiorano 64
00143 Rome
Italy

Tel.     +39.06 501703073 (office)
          +39.06 501703090  (lab)

Fax:      +39.06 501703326

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